概述

  • 产品名称
  • 描述
    山羊多克隆抗体to PPP2R4
  • 特异性
    This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Cow, Dog, Orangutan
  • 免疫原

    Synthetic peptide:

    C-GSLLPIHPVTSG

    , corresponding to C terminal amino acids 312-323 of Human PPP2R4 (NP_066954.2).

  • 阳性对照
    • Human, mouse or rat Liver lysate and HepG2 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab87673 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

靶标

  • 功能
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the PTPA-type PPIase family.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • subunit B'' antibody
    • KIAA0044 antibody
    • MGC2184 antibody
    • Phosphotyrosyl phosphatase activator antibody
    • PP2A antibody
    • PP2A phosphatase activator antibody
    • PP2A subunit B' antibody
    • PP2A subunit B' isoform PR53 antibody
    • PP2A subunit B' PR53 isoform antibody
    • PPP2R4 antibody
    • PR53 antibody
    • PR53 isoform antibody
    • Protein phosphatase 2, regulatory subunit B-prime antibody
    • Protein phosphatase 2A activator regulatory subunit 4 antibody
    • Protein phosphatase 2A regulatory subunit B' (PR 53) antibody
    • PTPA antibody
    • PTPA_HUMAN antibody
    • Serine/threonine protein phosphatase 2A regulatory subunit B' antibody
    • Serine/threonine-protein phosphatase 2A activator antibody
    • Serine/threonine-protein phosphatase 2A regulatory subunit 4 antibody
    • Serine/threonine-protein phosphatase 2A regulatory subunit B'' antibody
    see all

图片

  • All lanes : Anti-PPP2R4 antibody (ab87673) at 0.5 µg/ml

    Lane 1 : Human Liver lysate in RIPA buffer
    Lane 2 : Mouse Liver lysate in RIPA buffer
    Lane 3 : Rat Liver lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 37 kDa

实验方案

文献

ab87673 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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