All lanes : Anti-PPP2R3B antibody (ab72027) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 65 kDa Observed band size: 65 kDa Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of PPP2R3B.
Lane 1: ab72027 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
For western blot detection step ab72027 was used at 1 µg/ml.
Chemiluminescence with an exposure time of 30 seconds.