Anti-PPP2R1A抗体- Aminoterminal end (ab76697)

概述

  • 产品名称
    Anti-PPP2R1A抗体- Aminoterminal end
    参阅全部 PPP2R1A 一抗
  • 描述
    兔多克隆抗体to PPP2R1A - Aminoterminal end
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide selected from the N-terminal region of human PPP2R1A conjugated to KLH

  • 阳性对照
    • Jurkat, T47D, and HepG2 cell line lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab76697 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

靶标

  • 功能
    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
  • 序列相似性
    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.
  • 结构域
    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • 细胞定位
    Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2AAA_HUMAN antibody
    • 6330556D22Rik antibody
    • Alpha isoform of regulatory subunit A, protein phosphatase antibody
    • DKFZp470L0914 antibody
    • Medium tumor antigen-associated 61 kDa protein antibody
    • MGC128399 antibody
    • MGC786 antibody
    • MGC83000 antibody
    • MGC95083 antibody
    • PP2A Aalpha antibody
    • PP2A antibody
    • PP2A subunit A isoform PR65-alpha antibody
    • PP2A subunit A isoform R1-alpha antibody
    • PP2A, subunit A, PR65-alpha isoform antibody
    • PP2A, subunit A, R1-alpha isoform antibody
    • PP2AAALPHA antibody
    • Ppp2r1a antibody
    • ppp2r1a-b antibody
    • PR65 antibody
    • PR65-alpha antibody
    • PR65A antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody
    • Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A, antibody
    • Protein phosphatase 2 regulatory subunit A alpha antibody
    • Protein phosphatase 2, 65-KD regulatory subunit A, alpha antibody
    • Protein phosphatase 2, regulatory subunit A, alpha isoform antibody
    • Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody
    see all

图片

  • All lanes : Anti-PPP2R1A antibody - Aminoterminal end (ab76697) at 1/60 dilution

    Lane 1 : Jurkat cell line lysate
    Lane 2 : T47D cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab76697 has not yet been referenced specifically in any publications.

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