The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
应用说明Is unsuitable for IP.
功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro). Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
All lanes : Anti-PPP2CB antibody (ab72450) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 36 kDa Observed band size : 36 kDa Additional bands at : 100 kDa,28 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Anti-PPP2CB antibody (ab72450)参考文献
has not yet been referenced specifically in any publications.