概述

  • 产品名称Anti-PPP2CB抗体
    参阅全部 PPP2CB 一抗
  • 描述
    兔多克隆抗体to PPP2CB
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide from a region between residue 260 and the C-terminus (residue 309) of human PPP2CB, NP_002706.1.

  • 阳性对照
    • Whole cell lysate from Hela, NIH3T3 and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 28, 36 and 190 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
    • 序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
    • 翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • 细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • PP2A beta antibody
      • PP2A-beta antibody
      • PP2AB_HUMAN antibody
      • PP2Abeta antibody
      • PP2CB antibody
      • Ppp2cb antibody
      • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isozyme antibody
      • Protein phosphatase 2, catalytic subunit, beta isoform antibody
      • Protein phosphatase 2A catalytic subunit beta isoform antibody
      • Protein phosphatase type 2A catalytic subunit antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
      see all

    Anti-PPP2CB antibody 图像

    • All lanes : Anti-PPP2CB antibody (ab72343) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 190 kDa,28 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Immunoprecipitation/ Western Blot of PPP2CB.
      Lane 1: ab72343 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72343 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    Anti-PPP2CB antibody (ab72343)参考文献

    ab72343 has not yet been referenced specifically in any publications.

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