The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at a concentration of 4 - 5 µg/ml.
Note: Immunoprecipitates PP2A catalytic subunit from rabbit brain membrane extract and and 1mg human A431 RIPA cell lysate.
Phosphatase assay: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.
Note: Detects PP2A catalytic subunit (~34kD) in rabbit brain membrane cytosol and in human A431 RIPA cell lysate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro). Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.