概述

  • 产品名称Anti-PPP2CB抗体
    参阅全部 PPP2CB 一抗
  • 描述
    兔多克隆抗体to PPP2CB
  • 特异性ab32673 recognises PPP2CB. Does not crossreact with PP1 or other protein phosphatases
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Chicken, Cow, Dog, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Cynomolgus Monkey, Dictyostelium discoideum
  • 免疫原

    Synthetic peptide:

    GEPHVTRRTPDYFL

    conjugated to KLH, corresponding to C terminal amino acids 296/309 of Human PPP2CB.

  • 阳性对照
    • Rabbit brain membrane cytosol and human A431 RIPA cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab32673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at a concentration of 4 - 5 µg/ml.
    Note: Immunoprecipitates PP2A catalytic subunit from rabbit brain membrane extract and and 1mg human A431 RIPA cell lysate.
    Phosphatase assay: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.
    Note: Detects PP2A catalytic subunit (~34kD) in rabbit brain membrane cytosol and in human A431 RIPA cell lysate.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
    • 序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
    • 翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • 细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • PP2A beta antibody
      • PP2A-beta antibody
      • PP2AB_HUMAN antibody
      • PP2Abeta antibody
      • PP2CB antibody
      • Ppp2cb antibody
      • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isozyme antibody
      • Protein phosphatase 2, catalytic subunit, beta isoform antibody
      • Protein phosphatase 2A catalytic subunit beta isoform antibody
      • Protein phosphatase type 2A catalytic subunit antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
      see all

    Anti-PPP2CB antibody (ab32673)参考文献

    ab32673 has not yet been referenced specifically in any publications.

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