antigen: ELAQVKVFNA GDDPSVPLHV LSRLAINSDL CCSSQDLEFT LHCLVPDFPP PVEAADFGER LQRQLVCLER VTCSDL, corresponding to amino acids 112-187 of Human PPP1R3D (O95685).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis.
Expressed in all tissue tested. High expression in skeletal muscle and heart.
Contains 1 CBM21 (carbohydrate binding type-21) domain.
The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.
Information by UniProt
- PP1 subunit R6 antibody
- PPP1R3D antibody
- PPP1R6 antibody
Immunocytochemistry/ Immunofluorescence - Anti-PPP1R3D antibody (ab122696)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli, cytoplasm and mitochondria. Recommended concentration of ab122696 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3D antibody (ab122696)
ab122696, at 1/200 dilution, staining PPP1R3D in Paraffin-embedded Human rectum tissue by
has not yet been referenced specifically in any publications.
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