概述
- 产品名称Anti-PPP1R10抗体
参阅全部 PPP1R10 一抗 - 描述兔多克隆抗体to PPP1R10
- 经测试应用适用于: WB, IPmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish
- 免疫原
Other Immunogen Type corresponding to PPP1R10 (C terminal). A region between residue 900 and the C-terminus (residue 940) of human PPP1R10.
- 阳性对照
- Nuclear extract of Hela cells
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明Antibody was affinity purified using an epitope specific to PPP1R10 immobilized on solid support.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab70248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 99 kDa). | |
| IP | Use at 1-10 µg/mg of lysate. |
靶标
- 相关性PPP1R10 (serine/threonine protein phosphatase 1 regulatory subunit 10), also known as PNUTS, is a targeting subunit of protein phosphatase 1 (PP1). Furthermore, PPP1R10 acts as an inhibitor of PPP1CA and PPP1CC phosphatase activities. Its inhibitory activity on PPP1CA is only observed when PPP1R10 is phosphorylated by Protein Kinase A (PKA). PPP1R10 contains a zinc finger motif and is predominantly localised within the nucleus. Recently, PPP1R10 has been shown to target PP1 to GABA(C) receptors, shuttling from the nucleus to the cell membrane in cells co-expressing this receptor. This highlights a role for this protein in the regulation of synaptic signal transduction. PPP1R10 has also been shown to regulate the activity of p53 in response to hypoxia.
- 细胞定位Nucleus. Note: Found in discrete nucleoplasmic bodies and within nucleoli.
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数据库链接
- Entrez Gene: 462544 Chimpanzee
- Entrez Gene: 5514 Human
- Entrez Gene: 52040 Mouse
- Entrez Gene: 100144450 Pig
- Entrez Gene: 65045 Rat
- Entrez Gene: 711949 Rhesus monkey
- Omim: 603771 Human
- SwissProt: Q7YR38 Chimpanzee
see all -
别名
- CAT 53 antibody
- CAT53 antibody
- FB 19 antibody
see all
Anti-PPP1R10 antibody 图像
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Western blot - PPP1R10 antibody (ab70248)Lanes 1 - 2 : Anti-PPP1R10 antibody (ab70248) at 1 µg/ml
Lanes 3 - 4 : Anti-PPP1R10 antibody (ab70248) at 0.2 µg/ml
Lane 1 : Nuclear extracts from Hela cells at 15 µg
Lane 2 : Nuclear extracts from Hela cells at 45 µg
Lane 3 : Nuclear extracts from Hela cells at 15 µg
Lane 4 : Nuclear extracts from Hela cells at 45 µg
Predicted band size : 99 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - PPP1R10 antibody (ab70248)Immunoprecipitation/ Western Blot of PPP1R10
Lane 1: Another antibody which recognizes a different epitope of PPP1R10. Lane 2: Another antibody which recognizes a different epitope of PPP1R10.
Lane 3: Another antibody which recognizes a different epitope of PPP1R10. Lane 4: ab70248 at 1µg/100µg nuclear extract.
Detection: Chemiluminescence with exposure times of 3 seconds.
Anti-PPP1R10 antibody (ab70248)参考文献
ab70248 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"