Anti-PPME1抗体(ab86409)

概述

  • 产品名称Anti-PPME1抗体
    参阅全部 PPME1 一抗
  • 描述
    兔多克隆抗体to PPME1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (GRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHG G) of Human PPME1 (NP_057231)

  • 阳性对照
    • 721_B cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-PPME1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling PPME1 with ab86409 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - PPME1. Right - Merge.
  • Anti-PPME1 antibody (ab86409) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cortex tissue labelling PPME1 with ab86409 at 5µg/ml.

Anti-PPME1 antibody (ab86409)参考文献

ab86409 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (brain)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification brain
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"