概述

  • 产品名称Anti-PPM1D抗体
    参阅全部 PPM1D 一抗
  • 描述
    兔多克隆抗体to PPM1D
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PPM1D.

    (Peptide available as ab32657.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; HEK293 and in HeLa cell line in ICC/IF

性能

应用

Our Abpromise guarantee covers the use of ab31270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 23556002
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.
  • 序列相似性Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 3.1.3.16 antibody
    • p53 induced protein phosphatase 1 antibody
    • p53-induced protein phosphatase 1 antibody
    • PP2C delta antibody
    • PP2C-delta antibody
    • PP2CD antibody
    • Ppm1d antibody
    • PPM1D_HUMAN antibody
    • Protein phosphatase 1D antibody
    • protein phosphatase 1D magnesium-dependent delta isoform antibody
    • Protein phosphatase 2C delta isoform antibody
    • Protein phosphatase 2C isoform delta antibody
    • Protein phosphatase magnesium dependent 1 delta antibody
    • Protein phosphatase magnesium-dependent 1 delta antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1D antibody
    • Protein phosphatase Wip1 antibody
    • Wild type p53 induced phosphatase 1 antibody
    • WIP 1 antibody
    • WIP1 antibody
    see all

Anti-PPM1D antibody 图像

  • All lanes : Anti-PPM1D antibody (ab31270) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 67 kDa


    Exposure time : 20 minutes
  • Immunohistochemical staining using ab31720 in paraffin-embedded HCC and non-cancerous liver tissues, 5 µm sections, at a dilution of 1/1000. (a) Negative ppm1d staining in normal liver tissue. (b) Negative staining of HCC tissue without ab31720. (c) Ppm1d-positive non-cancerous liver tissue. (d) High expression of ppm1d in HCC tissue. Antigen retrieval was performed in a microwave with 0.1 M sodium citrate buffer (pH 6.0). The secondary antibody was a HRP conjugated compact polymer system and the samples were counter stained with DAB. These images were taken on a Keyence BZ-8000 digital microscope at a magnification of x200 and the scale bar represents 100 µm.

  • IHC image of ab31270 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31270, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab31270 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31270, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PPM1D antibody (ab31270)参考文献

This product has been referenced in:
  • Li GB  et al. Protein phosphatase magnesium-dependent 1d (PPM1D) mRNA expression is a prognosis marker for hepatocellular carcinoma. PLoS One 8:e60775 (2013). IHC-P ; Human . Read more (PubMed: 23556002) »
  • Zajkowicz A  et al. Nutlin-3a, an MDM2 antagonist and p53 activator, helps to preserve the replicative potential of cancer cells treated with a genotoxic dose of resveratrol. Mol Biol Rep 40:5013-26 (2013). WB ; Human . Read more (PubMed: 23666059) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Cell (Primary cells, testicular germ cells))
Permeabilization Yes - Triton X-100
Specification Mouse Cell (Primary cells, testicular germ cells)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Sep 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"