Anti-PPAR gamma抗体- ChIP Grade (ab45036)

概述

  • 产品名称Anti-PPAR gamma抗体- ChIP Grade
    参阅全部 PPAR gamma 一抗
  • 描述
    兔多克隆抗体to PPAR gamma - ChIP Grade
  • 特异性Ab45036 detects peroxisome proliferator activated receptor (PPAR) gamma 2. This antibody does not detect PPAR alpha or PPAR delta. This sequence is from P37231-1 (Isoform 2), the sequence is not present in P37231-2 (Isoform 1) or P37231-3 (Isoform 3).
  • 经测试应用适用于: ChIP, IP, IHC-P, ICC/IF, WB, Functional Studies, EMSA, ELISAmore details
  • 种属反应性
    与反应: Mouse, Cow, Human, Non Human Primates
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human PPAR gamma 2 aa 1-16.
    Sequence:

    MGETLGDSPIDPESDSC


    (Peptide available as ab41762)

  • 阳性对照
    • NIH-3T3 cell lysate

应用

Our Abpromise guarantee covers the use of ab45036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 21247904
IP Use at an assay dependent concentration. PubMed: 21247904
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.
WB 1/500. Predicted molecular weight: 56 kDa.Can be blocked with Human PPAR gamma peptide (ab41762).

By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.

Functional Studies Use at an assay dependent concentration. Ab45036 inhibits PPAR gamma 2 DNA binding.
EMSA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • 组织特异性Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • 疾病相关Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIMT1 antibody
    • GLM1 antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • OTTHUMP00000185032 antibody
    • OTTHUMP00000185036 antibody
    • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
    • Peroxisome proliferator activated receptor gamma 1 antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    • PPARgamma antibody
    see all

Anti-PPAR gamma antibody - ChIP Grade 图像

  • All lanes : Anti-PPAR gamma antibody - ChIP Grade (ab45036) at 1/500 dilution

    Lane 1 : 3T3
    Lane 2 : 3T3-L1 differentiated day 7

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 56 kDa

    PPAR gamma is detected in 3T3-L1 Day 7 differentiated cell lysates with some background bands. No detection of PPAR gamma occurs in 3T3 cell lysate.

     

  • Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. 3T3-L1 cells providing positive signal have been differentiated for 7 days.

    PPAR gamma is shown in red, lipid droplets (that indicates the proper differentiation of the cells) are shown in green.

  • Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.

     

  • Lane 1 :
    Lane 2 : Anti-PPAR gamma antibody - ChIP Grade (ab45036)

    Lane 1 : Protein Marker
    Lane 2 : Mouse liver tissue lysate at 1 µg

    Secondary
    Lane 2 : IRDye® 680RD Donkey anti-Rabbit


    Performed under reducing conditions.

    Predicted band size : 56 kDa


    Exposure time : 2 seconds

    This image is courtesy of Xiaofeng Xin

    See Abreview

Anti-PPAR gamma antibody - ChIP Grade (ab45036)参考文献

This product has been referenced in:

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Human Tissue sections (Adipose)
Specification Adipose
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 1 µg
Specification Liver
Gel Running Conditions Reduced Denaturing
Blocking step Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Dr. Xiaofeng Xin

Verified customer

提交于 May 13 2013

DISCOUNT CODE: 100%ABR-00000
Expiration date: 06. January 2013

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