概述

  • 产品名称Anti-PPAR delta抗体
    参阅全部 PPAR delta 一抗
  • 描述
    兔多克隆抗体to PPAR delta
  • 特异性This affinity purified antibody is directed against mouse PPAR delta protein.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    MEQPQEETPEAREE

    , corresponding to N terminal amino acids 1-14 of Mouse PPAR delta .

  • 阳性对照
    • 3T3 cell lysate HepG2
  • 常规说明

    OMIM link - 600409

性能

应用

Our Abpromise guarantee covers the use of ab8937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • 组织特异性Ubiquitous with maximal levels in placenta and skeletal muscle.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Anti-PPAR delta antibody 图像

  • ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 50 kDa

    Rabbit polyclonal to PPAR delta (ab8937).
    Each lane contains 20 ug 3T3 cell lysate

    Lane 1: ab8937 1/500
    Lane 2: ab8937 1/1000

    Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000

    Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000

Anti-PPAR delta antibody (ab8937)参考文献

This product has been referenced in:
  • Sharif O  et al. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog 10:e1004167 (2014). WB ; Mouse . Read more (PubMed: 24945405) »
  • Gatfield D  et al. Integration of microRNA miR-122 in hepatic circadian gene expression. Genes Dev 23:1313-26 (2009). IP ; Mouse . Read more (PubMed: 19487572) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Colorectal cancer HCT116)
Loading amount 10 µg
Specification Colorectal cancer HCT116
Gel Running Conditions Non-reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Injoo Hwang

Verified customer

提交于 Nov 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - nuclear (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2008

Thank you for the details that you have provided us with. This antibody has been characterized in Western blotting using 3T3 cell lysate, and we have not specifically tested it with human samples (although it is expected to cross-react due to sequence ...

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Thank you for your enquiry regarding ab8937. Unfortunately we don't supply Elisa Kits for this antibody. In the future, if you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway"....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"