Anti-PPAR delta抗体(ab8937)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PPAR delta抗体
参阅全部 PPAR delta 一抗 -
描述
兔多克隆抗体to PPAR delta -
宿主
Rabbit -
特异性
This affinity purified antibody is directed against mouse PPAR delta protein. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide:
MEQPQEETPEAREE
, corresponding to N terminal amino acids 1-14 of Mouse PPAR delta . -
阳性对照
- 3T3 cell lysate HepG2
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (3) |
1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
组织特异性
Ubiquitous with maximal levels in placenta and skeletal muscle. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Entrez Gene: 25682 Rat
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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别名
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
图片
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ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysateLane 1: ab8937 1/500
Lane 2: ab8937 1/1000Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000
Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab8937 被引用在 16 文献中.
- Liu Y et al. Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ. Nat Commun 13:2665 (2022). PubMed: 35562376
- Ho SY et al. Investigating the Role of PPARß/d in Retinal Vascular Remodeling Using Pparß/d-Deficient Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32575793
- Luo Y et al. The function of peroxisome proliferator-activated receptors PPAR-? and PPAR-d in Mycobacterium leprae-induced foam cell formation in host macrophages. PLoS Negl Trop Dis 14:e0008850 (2020). PubMed: 33075048
- Mandai S et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep 8:9101 (2018). PubMed: 29904119
- Hu X et al. MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL. Cell Physiol Biochem 47:545-555 (2018). PubMed: 29794473