Anti-PPAR delta抗体(ab66770)

概述

  • 产品名称Anti-PPAR delta抗体
    参阅全部 PPAR delta 一抗
  • 描述
    兔多克隆抗体to PPAR delta
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Cow, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PPAR delta .

    (Peptide available as ab68691.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Hydroxyurea Treated (48hr, 2µM), Rat Liver Tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • 组织特异性Ubiquitous with maximal levels in placenta and skeletal muscle.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Anti-PPAR delta antibody 图像

  • All lanes : Anti-PPAR delta antibody (ab66770) at 1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Anti-PPAR delta antibody (ab66770) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-PPAR delta antibody (ab66770)参考文献

ab66770 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

The immunogen sequence used to raise the anti-PPAR delta antibody (ab21209) is proprietary information. However, in order to aid your research I am able to share it with you. Ple...

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Thank you for your reply.

Indeed, the other antibodies mentioned are only tested for WB so far, however, they are the antibodies against these targets that have the highest probability to cross-react with hamster due to sequence similarity o...

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Thank you very much for contacting us.

I am happy to let you know that we have the following antibodies available:

COX-2: ab102005: Click here (or use the following: http://www.abcam.com/index.html?datasheet=102005).
COX-1: a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"