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Our Abpromise guarantee covers the use of ab23673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 4 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with Human PPAR delta peptide (ab104373). Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
Immunohistochemical analysis of paraffin-embedded pig kidney tissue labeling PPAR delta with ab23673 at 1/100 dilution,followed by Goat Anti-Rabbit IgG
ab23673 staining PPAR delta in Rat brain tissue (ab29475) sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100 in PBS) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit (ab150077) polyclonal (1/200) was used as the secondary antibody.
ab23673 staining mouse brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 48 hours at 4°C. A FITC conjugated goat anti-rabbit antibody was used as the secondary.
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