Anti-PPAR delta抗体[4E3-1B11] (ab58137)


  • 产品名称
    Anti-PPAR delta抗体[4E3-1B11]
    参阅全部 PPAR delta 一抗
  • 描述
    小鼠单克隆抗体[4E3-1B11] to PPAR delta
  • 经测试应用
    适用于: Flow Cyt, WB, ICC/IFmore details
  • 种属反应性
    与反应: Hamster, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-362 of Human PPAR delta



Our Abpromise guarantee covers the use of ab58137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µl for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 2.5 µg/ml.


  • 功能
    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • 组织特异性
    Ubiquitous with maximal levels in placenta and skeletal muscle.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Anti-PPAR delta antibody [4E3-1B11] 图像

  • Predicted band size : 50 kDa
    PPAR delta antibody (ab58137) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Overlay histogram showing JEG3 cells stained with ab58137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58137, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-PPAR delta antibody [4E3-1B11] (ab58137)参考文献

This product has been referenced in:
  • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . Read more (PubMed: 25969669) »
  • Kemmerer M  et al. AMP-Activated Protein Kinase Interacts with the Peroxisome Proliferator-Activated Receptor Delta to Induce Genes Affecting Fatty Acid Oxidation in Human Macrophages. PLoS One 10:e0130893 (2015). WB ; Human . Read more (PubMed: 26098914) »

See all 3 Publications for this product

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Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 ( as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. Howeve...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Hamster Cell (Expression of Human PPAR delta in CHO)
Expression of Human PPAR delta in CHO
Yes - TBS + 3% Dried Milk + 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C

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提交于 Jul 29 2008