Anti-PPAR alpha抗体[OTI1E8] (ab119416)

概述

  • 产品名称
    Anti-PPAR alpha抗体[OTI1E8]
    参阅全部 PPAR alpha 一抗
  • 描述
    小鼠单克隆抗体[OTI1E8] to PPAR alpha
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 145-420 of Human PPAR alpha.

  • 阳性对照
    • HEPG2 lysate, SVT2 lysate.
  • 常规说明

    The clone number has been updated from 1E8 to OTI1E8, both clone numbers name the same clone.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

应用

Our Abpromise guarantee covers the use of ab119416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/500. Predicted molecular weight: 52.2 kDa.
ICC/IF 1/100.

靶标

  • 功能
    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • 组织特异性
    Skeletal muscle, liver, heart and kidney.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

图片

  • All lanes : Anti-PPAR alpha antibody [OTI1E8] (ab119416)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control lysate
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PPARA lysate

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52.2 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216176)
  • All lanes : Anti-PPAR alpha antibody [OTI1E8] (ab119416)

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 52.2 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216176)
  • ab119416, staining PPAR alpha by immunocytochemistry on COS7 cells transiently transfected with pCMV6-ENTRY PPARA.

文献

ab119416 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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