Anti-PPAR alpha抗体(ab61182)
Key features and details
- Rabbit polyclonal to PPAR alpha
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PPAR alpha抗体
参阅全部 PPAR alpha 一抗 -
描述
兔多克隆抗体to PPAR alpha -
宿主
Rabbit -
特异性
ab61182 detects PPAR alpha protein, (SwissProt Q07869). -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic non-phosphopeptide derived from human PPAR alpha around the phosphorylation site of serine 21 (L-E-SP-P-L).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61182于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
1/100 - 1/500.
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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说明 |
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ICC/IF
1/100 - 1/500. |
WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
靶标
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功能
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
组织特异性
Skeletal muscle, liver, heart and kidney. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Omim: 170998 Human
- SwissProt: Q07869 Human
- SwissProt: P23204 Mouse
- Unigene: 103110 Human
- Unigene: 710044 Human
- Unigene: 212789 Mouse
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别名
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
图片
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ab61182 at 1/100 - 1/500 dilution staining PPAR alpha in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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All lanes : Anti-PPAR alpha antibody (ab61182) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts
Lane 2 : NIH 3T3 cell extracts with the immunising peptide
Predicted band size: 52 kDa
Observed band size: 52 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab61182 被引用在 6 文献中.
- Chen HX et al. Garcinia Biflavonoid 1 Improves Lipid Metabolism in HepG2 Cells via Regulating PPARα. Molecules 27:N/A (2022). PubMed: 35335339
- Mannino F et al. Atherosclerosis Plaque Reduction by Lycopene Is Mediated by Increased Energy Expenditure through AMPK and PPARα in ApoE KO Mice Fed with a High Fat Diet. Biomolecules 12:N/A (2022). PubMed: 35883529
- Wang X et al. Therapeutic Effects of Retinoic Acid in Lipopolysaccharide-Induced Cardiac Dysfunction: Network Pharmacology and Experimental Validation. J Inflamm Res 15:4963-4979 (2022). PubMed: 36105385
- Zhang B et al. Atorvastatin promotes AMPK signaling to protect against high fat diet-induced non-alcoholic fatty liver in golden hamsters. Exp Ther Med 19:2133-2142 (2020). PubMed: 32104276
- Li H et al. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond) 17:34 (2020). PubMed: 32377219
- Azar S et al. Reversal of diet-induced hepatic steatosis by peripheral CB1 receptor blockade in mice is p53/miRNA-22/SIRT1/PPARa dependent. Mol Metab 42:101087 (2020). PubMed: 32987186