Anti-PPAR alpha抗体(ab45859)

概述

  • 产品名称Anti-PPAR alpha抗体
    参阅全部 PPAR alpha 一抗
  • 描述
    兔多克隆抗体to PPAR alpha
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PPAR alpha.

    (Peptide available as ab46188.)

  • 阳性对照
    • This antibody gave a positive signal in the following Tissue Lystes: Mouse Brain Mouse Testis Mouse Heart Mouse Skeletal Muscle Rat Skeletal Muscle (data not shown)

应用

Our Abpromise guarantee covers the use of ab45859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • 组织特异性Skeletal muscle, liver, heart and kidney.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Anti-PPAR alpha antibody 图像

  • All lanes : Anti-PPAR alpha antibody (ab45859) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 47 kDa (possible isoform).

Anti-PPAR alpha antibody (ab45859)参考文献

ab45859 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Heart)
Loading amount 10 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 07 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Airway epithelial cells)
Specification Airway epithelial cells
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Prof. Marc Chanson

Verified customer

提交于 Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"