概述

  • 产品名称Anti-PPAP2A抗体
    参阅全部 PPAP2A 一抗
  • 描述
    兔多克隆抗体to PPAP2A
  • 特异性The immunogen for ab58745 is derived from a region specifically from isoform 2. This sequence shows low homology with isoform 1.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Guinea pig, Cat
  • 免疫原

    Synthetic peptide within Human PPAP2A aa 31-80 (N terminal). The exact sequence is proprietary. Isoform 2
    Sequence:

    QIYPFQRGFFCKDNSINYPYHDSTVTSTVLILVGVGLPISSIILGETLSV

  • 阳性对照
    • HepG2 cell lysate or human kidney tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab58745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). Pivotal regulator of lysophosphatidic acid (LPA) signaling in the cardiovascular system. Major enzyme responsible of dephosphorylating LPA in platelets, which terminates signaling actions of LPA. May control circulating, and possibly also regulate localized, LPA levels resulting from platelet activation. It has little activity towards ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > S-1-P > C-1-P. It's down-regulation may contribute to the development of colon adenocarcinoma.
  • 组织特异性Ubiquitously expressed with highest expression found in prostate. Isoform 1 is predominant in kidney, lung, placenta and liver. Isoform 2 is predominant in heart and pancreas. Found to be down-regulated in colon adenocarcinomas.
  • 序列相似性Belongs to the PA-phosphatase related phosphoesterase family.
  • 翻译后修饰N-glycosylated. Contains high-mannose oligosaccharides.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Lipid phosphate phosphohydrolase 1 antibody
    • Lipid phosphate phosphohydrolase 1a antibody
    • LLP1a antibody
    • LPP1 antibody
    • LPP1_HUMAN antibody
    • PAP 2a antibody
    • PAP-2a antibody
    • PAP2 antibody
    • PAP2-alpha antibody
    • PAP2a antibody
    • PAP2a2 antibody
    • PAP2alpha2 antibody
    • PAPalpha1 antibody
    • Phosphatidate phosphohydrolase type 2a antibody
    • Phosphatidic acid phosphatase 2a antibody
    • Phosphatidic acid phosphatase type 2A antibody
    • Phosphatidic acid phosphohydrolase type 2a antibody
    • PPAP2A antibody
    • Type 2 phosphatidic acid phosphatase alpha antibody
    • Type 2 phosphatidic acid phosphohydrolase antibody
    see all

Anti-PPAP2A antibody 图像

  • Anti-PPAP2A antibody (ab58745) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPAP2A with ab58745 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-PPAP2A antibody (ab58745)参考文献

This product has been referenced in:
  • Evseenko D  et al. Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironment. PLoS One 8:e63718 (2013). IHC-P ; Human . Read more (PubMed: 23696850) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"