Anti-PPAN抗体(ab122844)
Key features and details
- Rabbit polyclonal to PPAN
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-PPAN抗体 -
描述
兔多克隆抗体to PPAN -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: RWEMDRGRGR LCDQKFPKTK DKSQGAQARR GPRGASRDGG, corresponding to amino acids 421-460 of Human PPAN.
-
阳性对照
- RT4, U-251 MG and Human Tonsil lysates, Human oral mucosa tissue, U-251 MG cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122844于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
|
说明 |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
靶标
-
功能
May have a role in cell growth. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 Brix domain. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
-
数据库链接
- Entrez Gene: 56342 Human
- Omim: 607793 Human
- SwissProt: Q9NQ55 Human
- Unigene: 14468 Human
-
别名
- Brix domain containing protein 3 antibody
- Brix domain-containing protein 3 antibody
- BXDC3 antibody
see all
图片
-
All lanes : Anti-PPAN antibody (ab122844) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
ab122844 at 1/200 dilution staining PPAN in Paraffin-embedded Human oral mucosa tissue by Immunohistochemistry.
-
Immunofluorescent staining of Human cell line U-251 MG using ab122844 at a concentration of 4 µg/ml. ab122844 staining is shown in green.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab122844 被引用在 1 文献中.
- Keil M et al. Loss of Peter Pan protein is associated with cell cycle defects and apoptotic events. Biochim Biophys Acta Mol Cell Res 1866:882-895 (2019). PubMed: 30716409