概述

  • 产品名称
  • 描述
    兔多克隆抗体to PP5
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residue 1 and 50 of human PP5 (NP_006238.1).

  • 阳性对照
    • Whole cell lysate from Hela cells, 293T cells and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab71150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能
    May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
    Contains 3 TPR repeats.
  • 细胞定位
    Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ36922 antibody
    • FLJ55954 antibody
    • OTTHUMP00000165834 antibody
    • PP-T antibody
    • PP5 antibody
    • PPP5 antibody
    • PPP5_HUMAN antibody
    • Ppp5c antibody
    • PPT antibody
    • Protein phosphatase 5, catalytic subunit antibody
    • Protein phosphatase T antibody
    • Serine/threonine protein phosphatase 5 antibody
    • Serine/threonine-protein phosphatase 5 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PPP5 with ab71150 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PP5 antibody (ab71150) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of PP5
    Lane 1: ab71150 at 3µg/mg whole cell lysate.
    Lane 2: IgG control IP
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed using 1 µg/ml ab71150.
    Chemiluminescence with an exposure time of 5 seconds.

文献

ab71150 has not yet been referenced specifically in any publications.

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