概述

  • 产品名称Anti-PP5抗体
    参阅全部 PP5 一抗
  • 描述
    兔多克隆抗体to PP5
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human PP5.

    (Peptide available as ab27752.)

  • 阳性对照
    • This antibody gave a positive result in the following lysates: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue

性能

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应用

Our Abpromise guarantee covers the use of ab26053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).

靶标

  • 功能May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
    Contains 3 TPR repeats.
  • 细胞定位Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ36922 antibody
    • FLJ55954 antibody
    • OTTHUMP00000165834 antibody
    • PP-T antibody
    • PP5 antibody
    • PPP5 antibody
    • PPP5_HUMAN antibody
    • Ppp5c antibody
    • PPT antibody
    • Protein phosphatase 5, catalytic subunit antibody
    • Protein phosphatase T antibody
    • Serine/threonine protein phosphatase 5 antibody
    • Serine/threonine-protein phosphatase 5 antibody
    see all

Anti-PP5 antibody 图像

  • All lanes : Anti-PP5 antibody (ab26053) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PP5 antibody (ab26053) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa

Anti-PP5 antibody (ab26053)参考文献

This product has been referenced in:
  • Norris-Mullins B  et al. Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major. Parasite 21:25 (2014). WB . Read more (PubMed: 24890370) »
  • Zhang Y  et al. Estrogen inhibits glucocorticoid action via protein phosphatase 5 (PP5)-mediated glucocorticoid receptor dephosphorylation. J Biol Chem 284:24542-52 (2009). WB ; Human . Read more (PubMed: 19586900) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. The homology of the immunogen with Xenopus PP5 is 93%. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT1080 Cell line)
Specification HT1080 Cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
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提交于 Jun 04 2010

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount 50000 cells
Specification HT1080 Cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

提交于 Jun 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"