PP3,Negative control for PP2 (ab120617)
Key features and details
- Negative control for the Src kinase inhibitor PP2.
- CAS Number: 5334-30-5
- Purity: > 97%
- Soluble in ethanol to 25 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
PP3,Negative control for PP2 -
描述
Negative control for the Src kinase抑制剂PP2. -
生物学描述
Negative control for the Src kinase inhibitor PP2 (ab120308). Inhibits EGFR kinase (IC50 = 2.7 μM).
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纯度
> 97% -
CAS编号
5334-30-5 -
化学结构
性能
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化学名称
4-Amino-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine -
分子量
211.23 -
分子式
C11H9N5 -
PubChem识别号
4879 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in ethanol to 25 mM and in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=CC=C(C=C1)N2C3=C(C=N2)C(=NC=N3)N -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120617于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120617, PP3, Negative control for PP2
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ab11512 staining E cadherin in SW480 cells treated with PP3 (ab120617), by ICC/IF. No change of E cadherin expression with increased concentration of PP3 is observed, as described in literature, since PP3 is the negative control for PP2.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120617 (PP3) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (4)
ab120617 被引用在 4 文献中.
- Chen Z et al. Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway. Mediators Inflamm 2021:6639252 (2021). PubMed: 33927570
- Yoo JY et al. LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1. Cell Rep 33:108245 (2020). PubMed: 33086058
- Li C & Götz J Somatodendritic accumulation of Tau in Alzheimer's disease is promoted by Fyn-mediated local protein translation. EMBO J 36:3120-3138 (2017). PubMed: 28864542
- Lee EJ et al. Trans-synaptic zinc mobilization improves social interaction in two mouse models of autism through NMDAR activation. Nat Commun 6:7168 (2015). PubMed: 25981743