概述

  • 产品名称Anti-PP2D1抗体
  • 描述
    兔多克隆抗体to PP2D1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: NKSPSKGPLL FSTSEPNLTK SQSNIHVLFQ YKSVSEVRVS TTNSKENLSD SNYSKYCIYN PENVETFPAE TTHRKPCSEK VTDRPTSVND V, corresponding to amino acids 487-577 of Human PP2D1 (UniProt ID: A8MPX8).

  • 阳性对照
    • IHC-P: Human bone marrow tissue WB: RT-4, U-251 MG, Human liver and tonsil lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 数据库链接
  • 别名
    • C3orf48 antibody
    • PP2C-like domain-containing protein C3orf48 homolog antibody
    • PP2D1 antibody
    • PP2D1_HUMAN antibody
    • Protein phosphatase 2C-like domain containing 1 antibody
    • Protein phosphatase 2C-like domain-containing protein 1 antibody
    see all

Anti-PP2D1 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122375 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122375, at a 1/200 dilution, staining PP2D1 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.
  • All lanes : Anti-PP2D1 antibody (ab122375) at 1/250 dilution

    Lane 1 : Marker
    Lane 2 : RT-4 lysate
    Lane 3 : U-251 MG lysate
    Lane 4 : Human plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate

Anti-PP2D1 antibody (ab122375)参考文献

ab122375 has not yet been referenced specifically in any publications.

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