概述

  • 产品名称Anti-PP2A beta抗体
    参阅全部 PP2A beta 一抗
  • 描述
    兔多克隆抗体to PP2A beta
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to Human PP2A beta. Synthetic peptide from a region between residue 260 and the C-terminus (residue 309) of human PP2A beta

  • 阳性对照
    • Whole cell lysate from Hela, NIH3T3 and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 28, 36, 190 kDa (predicted molecular weight: 36 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
  • 序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
  • 翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • 细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PP2A beta antibody
    • PP2A-beta antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2CB antibody
    • Ppp2cb antibody
    • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isozyme antibody
    • Protein phosphatase 2, catalytic subunit, beta isoform antibody
    • Protein phosphatase 2A catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
    see all

Anti-PP2A beta antibody 图像

  • All lanes : Anti-PP2A beta antibody (ab72343) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 190 kDa,28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunoprecipitation/ Western Blot of PPP2CB.
    Lane 1: ab72343 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72343 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.

Anti-PP2A beta antibody (ab72343)参考文献

This product has been referenced in:
  • Joseph BK  et al. Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d. J Biol Chem 290:10588-98 (2015). Read more (PubMed: 25694423) »

See 1 Publication for this product

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