Anti-PP2A B subunit抗体(ab16448)
Key features and details
- Rabbit polyclonal to PP2A B subunit
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PP2A B subunit抗体 -
描述
兔多克隆抗体to PP2A B subunit -
宿主
Rabbit -
特异性
Based on sequence homology we believe this antibody will detect the following isoforms of the PP2A B subunit. The immunogen sequence is 100% identical to Q16537; epsilon isoform (PPP2R5E), Q13362; gamma isoform (PPP2R5C), Q14738; delta isoform (PPP2R5D) and Q15173 beta isoform (PPP2R5B), and 92% identical to Q15172; alpha isoform (PPP2R5A). If the isoform you are interested in is not listed, please contact our technical support team on technical@abcam.com who will be happy to check that for you.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human PP2A B subunit aa 352-365 conjugated to Keyhole Limpet Haemocyanin (KLH). The sequence is also 100% identical to Q13362 (PPP2R5C), Q14738 (PPP2R5D) and Q15173 (PPP2R5B), and 92% identical to Q15172 (PPP2R5A).
Sequence:VSSPHFQVAERALY
Database link: Q16537 -
阳性对照
- WB: Rat brain extract and Recombinant Human PPP2R5D protein (ab114547)
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常规说明
A protein phosphatase is a phosphatase enzyme that removes a phosphate group from the phosphorylated amino acid residue of its substrate protein. Protein phosphorylation is one of the most common forms of reversible protein posttranslational modification (PTM), with up to 30% of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyse the transfer of a γ-phosphate from ATP to specific amino acids on proteins. Several hundred PKs exist in mammals and are classified into distinct super-families. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 79.3, 16.9 and 3.8% respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. The largest class of PPs is the phosphoprotein phosphatase (PPP) family comprising PP1, PP2A, PP2B, PP4, PP5, PP6 and PP7, and the protein phosphatase Mg2+ or Mn2+-dependent (PPM) family, composed primarily of PP2C.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
Purified by Ammonium sulfate precipitation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16448于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
靶标
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相关性
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. There is an alpha isoform, a beta isoform, a gamma isoform, a delta isoform and an epsilon isoform. -
数据库链接
- SwissProt: Q13362 Human
- SwissProt: Q14738 Human
- SwissProt: Q15172 Human
- SwissProt: Q15173 Human
- SwissProt: Q16537 Human
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别名
- KIAA0044 antibody
- PP2A B subunit isoform B'-alpha antibody
- PP2A B subunit isoform B'-beta antibody
see all
数据表及文件
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Datasheet download
文献 (1)
ab16448 被引用在 1 文献中.
- Li P et al. Tristetraprolin attenuates brain edema in a rat model of cerebral hemorrhage. Brain Behav 9:e01187 (2019). PubMed: 30729695