RabMAb

Anti-PP2A alpha + beta抗体[E155] (ab32104)

概述

  • 产品名称Anti-PP2A alpha + beta抗体[E155]
    参阅全部 PP2A alpha + beta 一抗
  • 描述
    兔单克隆抗体[E155] to PP2A alpha + beta
  • 特异性The immunogen sequence for ab32104 has 100% homology with the PP2A-beta protein.
  • 经测试应用适用于: WB, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Zebrafish
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PP2A alpha + beta aa 250-350.

  • 阳性对照
    • IHC-P: Human pancreas tissue. WB: lysate of A431 cells (serum starved overnight) treated with EGF (100ng/ml) for 5-10 minutes at 37C (BD Biosciences).
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-PP2A-alpha  antibody (HRP) [E155] (ab196979)

    Anti-PP2A-alpha  antibody (Biotin) [E155] (ab197706)

性能

应用

Our Abpromise guarantee covers the use of ab32104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 35 kDa.
IHC-P Use at an assay dependent concentration.
IP 1/100.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • 序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
  • 翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • 细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

Anti-PP2A alpha + beta antibody [E155] 图像

  • All lanes : Anti-PP2A alpha + beta antibody [E155] (ab32104) at 1/5000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysates,cells were treated with Pervanadate for 30 minutes at the final concentration of 1mg/ml
    Lane 3 : HeLa (human cervix adenocarcinoma) whole cell lysates,cells were treated with Pervanadate for 30 minutes at the final concentration of 1mg/ml Then the membrane was incubated with Alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), HRP conjugated)

    Predicted band size : 35 kDa

    Blocking and Diluting buffer and concentration:5% NFDM/TBST

    Exposure time:3 minutes

  • ab32104 (2µg/ml) staining PP2A alpha  in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the islet of Langerhans.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Dot blot was performed using ab32104 at 1/1000 dilution.Goat Anti-Rabbit IgG,(H+L),HRP conjugated (ab97051) was used as secondary antibody at 1/100000 dilution

    Lane 1:PP2A alpha + beta  phospho peptide

    Lane 2:PP2A alpha + beta non-phospho peptide

    Blocking and Diluting buffer and concentration:5% NFDM/TBST

    Exposure time:10 seconds

  • ICC/IF image of ab32104 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32104, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PP2A alpha + beta antibody [E155] (ab32104) at 1/5000 dilution

    Lane 1 : untreated A431 cell lysate
    Lane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information)


    Predicted band size : 35 kDa
    Observed band size : 35 kDa

Anti-PP2A alpha + beta antibody [E155] (ab32104)参考文献

This product has been referenced in:
  • Inoue D  et al. SETBP1 mutations drive leukemic transformation in ASXL1-mutated MDS. Leukemia 29:847-57 (2015). Read more (PubMed: 25306901) »
  • Nardi F  et al. Enhanced insulin sensitivity associated with provision of mono and polyunsaturated fatty acids in skeletal muscle cells involves counter modulation of PP2A. PLoS One 9:e92255 (2014). Read more (PubMed: 24632852) »

See all 18 Publications for this product

Product Wall

Te pido disculpas por la espera.

Respecto al anticuerpo ab32104, hemos realizado un BLAST comparando ambas secuencias, y existe un 77% de homología entre ellas. No se prevé por tanto que el anticuerpo pueda reconocerla.
Read More

Thank you for your enquiry. I am sorry to hear you are having a problem with ab32104. To our knowledge, the antibody does not react with mouse. Therefore, we are unable to say, which of the two bands might be correct or if any of them is specific. ...

Read More

I have the regret to inform you that the scientist who tested the antibody informed me that the stimulation paradigm was confidential. I'm sorry I was unable to provide this information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"