Anti-PP2A alpha + beta抗体[0.T.118] (ab33537)

概述

  • 产品名称Anti-PP2A alpha + beta抗体[0.T.118]
    参阅全部 PP2A alpha + beta 一抗
  • 描述
    小鼠单克隆抗体[0.T.118] to PP2A alpha + beta
  • 特异性This antibody recognizes human PP2A, C subunit (PP2A alpha). It has been reported not to block phosphatase activity.
  • 经测试应用适用于: WB, IP, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Cow, Human, Xenopus laevis, Drosophila melanogaster
    预测可用于: Chicken, Dog, Pig, a wide range of other species
  • 免疫原

    Synthetic peptide corresponding to Human PP2A alpha + beta aa 295-309 (C terminal).
    Sequence:

    C-RGEPHVTRRTPDYFL

  • 阳性对照
    • WB: Human A431 and murine 3T3 RIPA cell lysates. IP: A431 RIPA lysate.

性能

应用

Our Abpromise guarantee covers the use of ab33537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP Use a concentration of 4 µg/ml.
ICC Use a concentration of 5 - 10 µg/ml. Fix for 10 minutes with 4% paraformaldehyde in PBS.

靶标

  • 功能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • 序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
  • 翻译后修饰Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • 细胞定位Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

Anti-PP2A alpha + beta antibody [0.T.118] (ab33537)参考文献

This product has been referenced in:
  • Ogawa H  et al. Protein phosphatase 2A negatively regulates aPKC signaling by modulating phosphorylation of Par-6 in Drosophila neuroblast asymmetric divisions. J Cell Sci 122:3242-9 (2009). IP ; Drosophila melanogaster . Read more (PubMed: 19690050) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam. Although we have eleven PP2A alpha antibodies, none of these have been raised in sheep and therefore none will be recognized by an anti-sheep IgG secondary antibody. SHould you decide to use ab33537 in your studies...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse adipocyte)
Loading amount 5 µg
Specification mouse adipocyte
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 May 16 2011

Thank you for your enquiry. We currently have two PP2A C antibodies in our catalogue (ab1180, ab33537) and I have enquired at the originator of the product for more information. I have listed them below for your reference. ab1180 This product d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"