Anti-PP2A-alpha抗体(ab106262)
Key features and details
- Rabbit polyclonal to PP2A-alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-PP2A-alpha抗体
参阅全部 PP2A-alpha 一抗 -
描述
兔多克隆抗体to PP2A-alpha -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
|
IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.
|
说明 |
---|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required. |
靶标
-
功能
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. -
序列相似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
翻译后修饰
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
细胞定位
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
-
数据库链接
- Entrez Gene: 5515 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 24672 Rat
- Omim: 176915 Human
- SwissProt: P67775 Human
- SwissProt: P63330 Mouse
- SwissProt: P63331 Rat
- Unigene: 105818 Human
see all -
别名
- PP2A alpha antibody
- PP2A-alpha antibody
- PP2AA_HUMAN antibody
see all
图片
-
All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/ml
Lane 1 : Rat kidney tissue Lysate
Lane 2 : Rat Liver tissue Lysate
Lane 3 : Human Placenta tissue Lysate
Lane 4 : Mouse Kidney tissue Lysate
Lane 5 : A431 Whole Cell Lysate
Lane 6 : HeLa Whole Cell Lysate
Lane 7 : 293T Whole Cell Lysate
Lane 8 : NIH Whole Cell Lysate
Lane 9 : PC12 Whole Cell Lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa -
Cytoplasmic staining of PP2A in a formalin fixed, paraffin embedded section of human mammary cancer, using ab106262 at a concentration of 2µg/ml.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (10)
ab106262 被引用在 10 文献中.
- Gao R et al. Protein phosphatase 2A catalytic subunit β suppresses PMA/ionomycin-induced T-cell activation by negatively regulating PI3K/Akt signaling. FEBS J 289:4518-4535 (2022). PubMed: 35068054
- Liang J et al. The effect of PPP2CA expression on the prognosis of patients with hepatocellular carcinoma and its molecular biological characteristics. J Gastrointest Oncol 12:3008-3021 (2021). PubMed: 35070426
- Liang Y et al. PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production. Cell Death Differ 28:2728-2744 (2021). PubMed: 33934104
- Lu Q et al. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease. JCI Insight 6:N/A (2021). PubMed: 34622800
- Yang CL et al. Potential Role and Clinical Value of PPP2CA in Hepatocellular Carcinoma. J Clin Transl Hepatol 9:661-671 (2021). PubMed: 34722181
- Mazhar S et al. Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac. Cell Rep 30:3164-3170.e3 (2020). PubMed: 32130915
- Leonard D et al. Selective PP2A Enhancement through Biased Heterotrimer Stabilization. Cell 181:688-701.e16 (2020). PubMed: 32315618
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). PubMed: 30969990
- Umesalma S et al. RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growth. J Clin Invest 130:N/A (2019). PubMed: 30721156
- Yi H et al. Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury via Modulating PKC-ß/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways. Antioxid Redox Signal 30:1949-1968 (2019). PubMed: 30484323