概述

  • 产品名称PP2
  • 描述
    Src and RIP2 kinase inhibitor
  • 生物学描述Inhibitor of the Src family of kinases. Inhibits Src and Lck kinase (IC50 values are 36 and 31 nM, respectively). Also shown to inhibit RIP2 and CK1δ (IC50 values are 19 and 41 nM, respectively).
  • 纯度> 98%

性能

  • 化学名称1-tert-Butyl-3-(4-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
  • 分子量301.78
  • 化学结构Chemical Structure
  • 分子式C15H16ClN5
  • CAS编号172889-27-9
  • PubChem识别号4878
  • 存放说明Store at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • 溶解度概述Soluble in DMSO to 50 mM and in ethanol to 10 mM
  • 处理

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESClc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C
  • 来源

    Synthetic

  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab120308 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    PP2 图像

    • ab11512 staining E-Cadherin in SW480 cells treated with PP2 (ab120308), by ICC/IF. Increase in E-cadherin expression correlates with increased concentration of PP2, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120308 (PP2) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab96971) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    PP2 (ab120308)参考文献

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