Anti-PP14397抗体(ab22843)

概述

  • 产品名称Anti-PP14397抗体
    参阅全部 PP14397 一抗
  • 描述
    兔多克隆抗体to PP14397
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from a region between residues 84-138 of Human PP14397.

  • 阳性对照
    • Jurkat cell lysate (western blot) and human skeletal muscle tissue slide (IHC-P).

性能

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应用

Our Abpromise guarantee covers the use of ab22843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 5 µg/ml. Detects a band of approximately 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

Anti-PP14397 antibody 图像

  • ab22843 staining human muscle tissue sections by IHC-P.  The primary antibody was diluted 4 µg/ml.  Arrows indicate positively labelled skeletal muscle cells.

  • Anti-PP14397 antibody (ab22843) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti rabbit IgG

Anti-PP14397 antibody (ab22843)参考文献

ab22843 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 30 µg
Specification 293T
Treatment siRNA
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
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提交于 Feb 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"