Anti-PP11抗体(ab121367)

概述

  • 产品名称Anti-PP11抗体
    参阅全部 PP11 一抗
  • 描述
    兔多克隆抗体to PP11
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Recombinant fragment, corresponding to amino acids 116-253 of Human PP11 (P21128).

  • 阳性对照
    • Human placenta tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab121367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
  • 组织特异性Placental-specific, but also associated with various malignant neoplasms.
  • 序列相似性Belongs to the ENDOU family.
    Contains 2 SMB (somatomedin-B) domains.
  • 翻译后修饰It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 22 serine protease antibody
    • 26 serine protease antibody
    • Endonuclease, polyU-specific antibody
    • endou antibody
    • ENDOU_HUMAN antibody
    • P11 antibody
    • Placental protein 11 antibody
    • Poly(U)-specific endoribonuclease antibody
    • PP11 antibody
    • Pp11r antibody
    • Protein endoU antibody
    • PRSS26 antibody
    • Uridylate-specific endoribonuclease antibody
    see all

Anti-PP11 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121367 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunocytochemical immunofluorescence analysis of methanol-fixed human HEK293T cells, labelling PP11 with 121367 at a dilution of 1/100 incubated for 16 hours at 4°C in 1% BSA in PBS with 0.1% Tween20 diluent. Blocking was with 1% BSA incubated for 1 hour at 25°C. Secondary was a goat anti-rabbit polyclonal Rhodamine conjugate at 1/100.

    See Abreview

  • ab121367 at 1/250 dilution staining PP11 in Paraffin-emebedded Human placenta tissue by Immunohistochemistry.

Anti-PP11 antibody (ab121367)参考文献

ab121367 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293T)
Permeabilization No
Specification HEK293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Methanol
Username

Mr. Chi-Cheng Hsieh

Verified customer

提交于 Nov 05 2015

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification HEK293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Chi-Cheng Hsieh

Verified customer

提交于 Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"