Anti-PP11抗体(ab121367)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PP11
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Recombinant fragment, corresponding to amino acids 116-253 of Human PP11 (P21128).

  • 阳性对照
    • Human placenta tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
  • 组织特异性
    Placental-specific, but also associated with various malignant neoplasms.
  • 序列相似性
    Belongs to the ENDOU family.
    Contains 2 SMB (somatomedin-B) domains.
  • 翻译后修饰
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 22 serine protease antibody
    • 26 serine protease antibody
    • Endonuclease, polyU-specific antibody
    • endou antibody
    • ENDOU_HUMAN antibody
    • P11 antibody
    • Placental protein 11 antibody
    • Poly(U)-specific endoribonuclease antibody
    • PP11 antibody
    • Pp11r antibody
    • Protein endoU antibody
    • PRSS26 antibody
    • Uridylate-specific endoribonuclease antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121367 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunocytochemical immunofluorescence analysis of methanol-fixed human HEK293T cells, labelling PP11 with 121367 at a dilution of 1/100 incubated for 16 hours at 4°C in 1% BSA in PBS with 0.1% Tween20 diluent. Blocking was with 1% BSA incubated for 1 hour at 25°C. Secondary was a goat anti-rabbit polyclonal Rhodamine conjugate at 1/100.

    See Abreview

  • ab121367 at 1/250 dilution staining PP11 in Paraffin-emebedded Human placenta tissue by Immunohistochemistry.

文献

ab121367 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
No
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Methanol
Username

Chi-Cheng Hsieh

Verified customer

提交于 Nov 05 2015

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Chi-Cheng Hsieh

Verified customer

提交于 Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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