The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
POU6F2 (POU class 6 homeobox 2) is a member of the POU domain family of transcription factors. It recognizes and binds to the DNA sequence 5'-ATGCAAAT-3' and may be involved in the early steps in the differentiation of amacrine and ganglion cells.