Anti-POU2F3抗体(ab101724)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to POU2F3
  • 宿主
    Goat
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide, corresponding to internal sequence amino acids 161-175 (

    EPHLEASQHLPVPKH

    ) of Human POU2F3 (NP_055167.2).

  • 阳性对照
    • HEK293 and HepG2 cell lines.

性能

应用

Our Abpromise guarantee covers the use of ab101724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.

靶标

  • 功能
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.
  • 组织特异性
    Specifically expressed in epidermis and cultured keratinocytes.
  • 序列相似性
    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • class 2 antibody
    • Epoc 1 antibody
    • Epoc1 antibody
    • HGNC19864 antibody
    • Oct 11 antibody
    • Oct-11 antibody
    • OCT11 antibody
    • Octamer binding transcription factor 11 antibody
    • Octamer-binding protein 11 antibody
    • Octamer-binding transcription factor 11 antibody
    • OTF-11 antibody
    • PLA 1 antibody
    • PLA1 antibody
    • PLA1 protein antibody
    • PO2F3_HUMAN antibody
    • POU class 2 homeobox 3 antibody
    • POU domain antibody
    • POU domain transcription factor OCT11a antibody
    • POU domain, class 2, transcription factor 3 antibody
    • POU transcription factor antibody
    • POU2F3 antibody
    • Skn 1a antibody
    • transcription factor 3 antibody
    • Transcription factor PLA-1 antibody
    • Transcription factor Skn-1 antibody
    • Transcription factor Skn1 antibody
    see all

图片

  • Anti-POU2F3 antibody (ab101724) at 2 µg/ml + HEK293 lysate in RIPA buffer at 35 µg

    Predicted band size: 47 kDa



    Incubation of ab101724 was 1 hour. Detected by chemiluminescence.

实验方案

文献

ab101724 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer, EDTA
Permeabilization
No
Specification
skin
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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提交于 Mar 12 2018

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