概述

  • 产品名称Anti-POT1抗体[EPR6319]
    参阅全部 POT1 一抗
  • 描述
    兔单克隆抗体[EPR6319] to POT1
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human POT1.

  • 阳性对照
    • K562, HepG2, 293T, and HeLa cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the telombin family.
    • 细胞定位Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZP586D211 antibody
      • hPot 1 antibody
      • hPot1 antibody
      • POT 1 antibody
      • POT1 antibody
      • POT1 like telomere end-binding protein antibody
      • POT1 protection of telomeres 1 homolog antibody
      • POT1-like telomere end-binding protein antibody
      • POTE1_HUMAN antibody
      • Protection of telomeres 1 antibody
      • Protection of telomeres 1 homolog (S. pombe) antibody
      • Protection of telomeres protein 1 antibody
      see all

    Anti-POT1 antibody [EPR6319] 图像

    • All lanes : Anti-POT1 antibody [EPR6319] (ab124784) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 71 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-POT1 antibody [EPR6319] (ab124784)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (LCL cells)
    Loading amount 30 µg
    Specification LCL cells
    Gel Running Conditions Reduced Denaturing (10% SDS)
    Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    提交于 Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"