概述

  • 产品名称
  • 描述
    兔多克隆抗体to POT1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length native protein (purified) (Human)

  • 阳性对照
    • U2OS cells.

性能

应用

Our Abpromise guarantee covers the use of ab90552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 71 kDa.
IP Use at an assay dependent dilution.

靶标

  • 功能
    Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the telombin family.
  • 细胞定位
    Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586D211 antibody
    • hPot 1 antibody
    • hPot1 antibody
    • POT 1 antibody
    • POT1 antibody
    • POT1 like telomere end-binding protein antibody
    • POT1 protection of telomeres 1 homolog antibody
    • POT1-like telomere end-binding protein antibody
    • POTE1_HUMAN antibody
    • Protection of telomeres 1 antibody
    • Protection of telomeres 1 homolog (S. pombe) antibody
    • Protection of telomeres protein 1 antibody
    see all

文献

ab90552 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Bone Osteosarcoma (U2OS))
Permeabilization
Yes - 0.3% Triton X-100
Specification
Human Bone Osteosarcoma (U2OS)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
PFA or MeOH
Username

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Verified customer

提交于 May 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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