概述

  • 产品名称Anti-POT1抗体
    参阅全部 POT1 一抗
  • 描述
    兔多克隆抗体to POT1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog, Chimpanzee, Monkey
  • 免疫原

    A portion of amino acids 450-500 of human POT1.

  • 阳性对照
    • HL60 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab47082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).

靶标

  • 功能Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the telombin family.
  • 细胞定位Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586D211 antibody
    • hPot 1 antibody
    • hPot1 antibody
    • POT 1 antibody
    • POT1 antibody
    • POT1 like telomere end-binding protein antibody
    • POT1 protection of telomeres 1 homolog antibody
    • POT1-like telomere end-binding protein antibody
    • POTE1_HUMAN antibody
    • Protection of telomeres 1 antibody
    • Protection of telomeres 1 homolog (S. pombe) antibody
    • Protection of telomeres protein 1 antibody
    see all

Anti-POT1 antibody 图像

Anti-POT1 antibody (ab47082)参考文献

ab47082 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Smooth muscle, HeLa, Jerkat)
Loading amount 10 µg
Specification Smooth muscle, HeLa, Jerkat
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tze Vun Liew

Verified customer

提交于 Jul 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"