Porcine PDGF-BB ELISA (ab218297)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig
概述
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产品名称
Porcine PDGF-BB ELISA
参阅全部 PDGF B 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 < 10% 批次间 样品 n Mean SD CV% 1 < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
10 pg/ml -
范围
10 pg/ml - 2500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 120.5 108% - 130% Serum 108.6 87% - 123% Plasma 112.3 98% - 115% -
检测时间
4h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human, Pig -
产品概述
The Porcine PDGF-BB ELISA kit (ab218297) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of porcine PDGF-BB in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X Streptavidin-Conjugated HRP 1 x 200µl Anti-Human PDGF-BB coated Microplate (12 x 8 wells) 1 x 96 tests Biotinylated (PDGF-BB) Detection Antibody 2 vials Lyophilized (PDGF-BB) Standard 2 vials Stop Solution 1 x 8ml TMB Substrate 1 x 12ml -
研究领域
- Information by UniProt
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别名
- Becaplermin
- c sis
- FLJ12858
see all -
数据库链接
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P20034 Pig
- Unigene: 1976 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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