Anti-PON3抗体[5G11] (ab71994)

概述

  • 产品名称Anti-PON3抗体[5G11]
    参阅全部 PON3 一抗
  • 描述
    小鼠单克隆抗体[5G11] to PON3
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Recombinant human protein purified from E.coli (His-PON3)

  • 阳性对照
    • HepG2 and Hep3B cell lysates

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab71994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
  • 序列相似性Belongs to the paraoxonase family.
  • 翻译后修饰The signal sequence is not cleaved.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810004E20 antibody
    • AI786302 antibody
    • MGC95026 antibody
    • Paraoxonase 3 antibody
    • Pon3 antibody
    • PON3_HUMAN antibody
    • Serum paraoxonase/lactonase 3 antibody
    see all

Anti-PON3 antibody [5G11] 图像

  • All lanes : Anti-PON3 antibody [5G11] (ab71994) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hep3B cell lysate


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • IHC image of ab71994 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71994, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PON3 antibody [5G11] (ab71994)参考文献

ab71994 has not yet been referenced specifically in any publications.

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