概述

  • 产品名称Anti-PON2抗体[AF3E6]
    参阅全部 PON2 一抗
  • 描述
    小鼠单克隆抗体[AF3E6] to PON2
  • 特异性We do not recommend to use this antibody in mouse and rat as very weak staining is observed.
  • 经测试应用适用于: Flow Cyt, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    His tagged recombinant Human PON2 protein purified from E.coli

  • 阳性对照
    • HeLa, HepG2 and 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1µg for 106 cells.
WB 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 31 kDa). We recommend to use milk rather than BSA to block. BSA can sometimes give high background with this antibody.
IP Use at an assay dependent concentration.

靶标

  • 功能Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
  • 组织特异性Widely expressed with highest expression in liver, lung, placenta, testis and heart.
  • 序列相似性Belongs to the paraoxonase family.
  • 翻译后修饰The signal sequence is not cleaved.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A esterase 2 antibody
    • A-esterase 2 antibody
    • Aromatic esterase 2 antibody
    • Paraoxonase 2 antibody
    • Paraoxonase nirs antibody
    • PON 2 antibody
    • PON2 antibody
    • PON2_HUMAN antibody
    • Serum aryldialkylphosphatase 2 antibody
    • Serum paraoxonase/arylesterase 2 antibody
    see all

Anti-PON2 antibody [AF3E6] 图像

  • Overlay histogram showing HepG2 cells stained with ab85340 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85340, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate


    Predicted band size : 31 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

    Lane 1 : PON1 Recombinant Protein
    Lane 2 : PON2 Recombinant Protein
    Lane 3 : PON3 Recombinant Protein
    Lane 4 : HeLa Cell Lysate
    Lane 5 : HepG2 Cell Lysate
    Lane 6 : U87mg Cell Lysate


    Predicted band size : 31 kDa
    Observed band size : 31,41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

    Lane 1 : HeLa Cell Lysate
    Lane 2 : PC12 Cell Lysate
    Lane 3 : H9C2 Cell Lysate
    Lane 4 : C6 Cell Lysate
    Lane 5 : L929 Cell Lysate
    Lane 6 : NIH3T3 Cell Lysate
    Lane 7 : Rat Liver Lysate
    Lane 8 : Mouse Liver Lysate

    Secondary
    Mouse-specific secondary at 1/5000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31,41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-PON2 antibody [AF3E6] (ab85340)参考文献

This product has been referenced in:
  • Mandrich L  et al. An Engineered Version of Human PON2 Opens the Way to Understand the Role of Its Post-Translational Modifications in Modulating Catalytic Activity. PLoS One 10:e0144579 (2015). WB ; Human . Read more (PubMed: 26656916) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"