概述

  • 产品名称
  • 描述
    兔多克隆抗体to PON2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Turkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON2.

    (Peptide available as ab41543.)

  • 阳性对照
    • This antibody gave a positive result in the following lysates: Caco2 whole cell, HeLa (Staurosporine treated) and HeLa (Hydroxyurea treated)

性能

应用

Our Abpromise guarantee covers the use of ab41544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

靶标

图片

  • All lanes : Anti-PON2 antibody (ab41544) at 1 µg/ml

    Lane 1 : Caco 2 whole cell lysate (ab3950)
    Lane 2 : HeLa - Staurosporine Treated (24hr, 500nM)
    Lane 3 : HeLa - Hydroxyurea Treated (48hr, 1µM)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 44 kDa (possible glycosylated form).

实验方案

文献

ab41544 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Dr. Sophie Pezet

Verified customer

提交于 May 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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