重组Anti-PON1抗体[EPR2892] (ab92466)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2892] to PON1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-PON1抗体[EPR2892]
参阅全部 PON1 一抗 -
描述
兔单克隆抗体[EPR2892] to PON1 -
宿主
Rabbit -
特异性
Mouse species is recommended based on IHC results, we do not guarantee WB for mouse.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human PON1 aa 50-150 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: Human plasma cell lysate and ovary carcinoma tissue lysate; IHC-P: Human liver tissue. Mouse liver, kidney, and colon tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR2892 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. -
组织特异性
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. -
疾病相关
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. -
序列相似性
Belongs to the paraoxonase family. -
翻译后修饰
Glycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 5444 Human
- Entrez Gene: 18979 Mouse
- Omim: 168820 Human
- SwissProt: P27169 Human
- SwissProt: P52430 Mouse
- Unigene: 370995 Human
- Unigene: 237657 Mouse
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别名
- A esterase 1 antibody
- A-esterase 1 antibody
- Aromatic esterase 1 antibody
see all
图片
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All lanes : Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution
Lane 1 : Human plasma cell lysate
Lane 2 : ovarian cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Lane 2 : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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ab92466, at 1/100 dilution, staining PON1 in formalin-fixed, paraffin-embedded Human liver tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab92466 showing negative staining in Skeletal muscle tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab92466 showing negative staining in Normal colon tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab92466 showing negative staining in Normal breast tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab92466 showing negative staining in Normal brain tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab92466 被引用在 4 文献中.
- Cao X et al. Analysis of Serum Paraoxonase 1 Using Mass Spectrometry and Lectin Immunoassay in Patients With Alpha-Fetoprotein Negative Hepatocellular Carcinoma. Front Oncol 11:651421 (2021). PubMed: 33889548
- Xu D et al. Identification and clinical validation of EMT-associated prognostic features based on hepatocellular carcinoma. Cancer Cell Int 21:621 (2021). PubMed: 34819088
- Ahn SB et al. Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel. Clin Proteomics 16:34 (2019). PubMed: 31467500
- Yu Z et al. Evaluation of the prognostic value of paraoxonase 1 in the recurrence and metastasis of hepatocellular carcinoma and establishment of a liver-specific predictive model of survival. J Transl Med 16:327 (2018). PubMed: 30477582