概述

  • 产品名称Anti-PON1抗体
    参阅全部 PON1 一抗
  • 描述
    兔多克隆抗体to PON1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PON1.

  • 阳性对照
    • This antibody gave a positive signal in Human Liver tissue lysate and human liver FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab113537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • 组织特异性Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
  • 疾病相关Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
  • 序列相似性Belongs to the paraoxonase family.
  • 翻译后修饰Glycosylated.
    The signal sequence is not cleaved.
    Present in two forms, form B contains a disulfide bond, form A does not.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A esterase 1 antibody
    • A-esterase 1 antibody
    • Aromatic esterase 1 antibody
    • Arylesterase 1 antibody
    • Arylesterase B type antibody
    • ESA antibody
    • Esterase A antibody
    • K 45 antibody
    • K-45 antibody
    • MVCD5 antibody
    • Paraoxonase 1 antibody
    • Paraoxonase antibody
    • Paraoxonase B type antibody
    • Paraoxonase, plasma antibody
    • Paraoxonase1 antibody
    • PON 1 antibody
    • PON antibody
    • PON1 antibody
    • PON1_HUMAN antibody
    • Serum aryldiakylphosphatase antibody
    • Serum aryldialkylphosphatase 1 antibody
    • Serum paraoxonase/arylesterase 1 antibody
    see all

Anti-PON1 antibody 图像

  • Anti-PON1 antibody (ab113537) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds
  • IHC image of PON1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113537, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PON1 antibody (ab113537)参考文献

ab113537 has not yet been referenced specifically in any publications.

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