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Our Abpromise guarantee covers the use of ab32893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
This product appears to react with the full length precursor protein only.
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/75. PubMed: 23469282|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21526203|
ab32893 (4µg/ml) staining of POMC in paraffin embedded Human Adrenal Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows vesiculate staining in some glandular cells.
ab32893 at 2.5ug/ml staining of paraffin embedded human adrenal gland tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.