Anti-POMC抗体[2F6H11] (ab73092)

概述

  • 产品名称Anti-POMC抗体[2F6H11]
    参阅全部 POMC 一抗
  • 描述
    小鼠单克隆抗体[2F6H11] to POMC
  • 经测试应用适用于: ELISA, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Pig
  • 免疫原

    Synthetic peptide:

    MPRSCCSRSGALLLALLLQASMEV

    conjugated to KLH, corresponding to amino acids 1-24 of Human POMC.

性能

应用

Our Abpromise guarantee covers the use of ab73092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • Information by UniProt
  • 数据库链接
  • 别名
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • alpha melanocyte stimulating hormone antibody
    • Alpha MSH antibody
    • Alpha-MSH antibody
    • beta endorphin antibody
    • Beta LPH antibody
    • beta melanocyte stimulating hormone antibody
    • Beta MSH antibody
    • Beta-LPH antibody
    • Beta-MSH antibody
    • CLIP antibody
    • COLI_HUMAN antibody
    • Corticotropin like intermediary peptide antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-lipotropin antibody
    • Gamma MSH antibody
    • Gamma-LPH antibody
    • Gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • LPH antibody
    • Melanotropin alpha antibody
    • Melanotropin antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • met enkephalin antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • POC antibody
    • POMC antibody
    • pro ACTH endorphin antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    see all

Anti-POMC antibody [2F6H11] 图像

  • Overlay histogram showing HeLa cells stained with ab73092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73092, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-POMC antibody [2F6H11] (ab73092)参考文献

ab73092 has not yet been referenced specifically in any publications.

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The JCN guidelines make a lot of sense to me and to my colleagues in the scientific support department. Abcam has a large catalogue of antibodies, and that has been one of the objectives from the start, to offer more antibodies than other companies. Ho...

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The antibody ab73092 (anti-POMC) has been successfully characterised by IHC using frozen and paraffin embedded sections. It has not been tested with free floating sections, but it is very likely to work in this ap...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"