Anti-polyHistidine抗体[HIS-1] (HRP) (ab49781)

概述

  • 产品名称
    Anti-polyHistidine抗体[HIS-1] (HRP)
    参阅全部 polyHistidine 一抗
  • 描述
    小鼠单克隆抗体[HIS-1] to polyHistidine (HRP)
  • 偶联物
    HRP
  • 特异性
    This antibody recognizes native as well as denatured, reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • 经测试应用
    适用于: ELISA, Dot blot, WBmore details
  • 免疫原

    Recombinant polyHistidine tagged fusion protein.

  • 阳性对照
    • Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
  • 常规说明

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

    The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
  • 克隆
    单克隆
  • 克隆编号
    HIS-1
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Dot blot 1/2000.
WB 1/2000.

靶标

  • 相关性
    Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

实验方案

Anti-polyHistidine antibody [HIS-1] (HRP) (ab49781)参考文献

This product has been referenced in:
  • Kim YJ  et al. Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28273846) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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