Anti-polyHistidine抗体[HIS-1] (HRP) (ab49781)

概述

  • 产品名称Anti-polyHistidine抗体[HIS-1] (HRP)
    参阅全部 polyHistidine 一抗
  • 描述
    小鼠单克隆抗体[HIS-1] to polyHistidine (HRP)
  • 偶联物HRP
  • 特异性This antibody recognizes native as well as denatured, reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • 经测试应用适用于: ELISA, Dot blot, WBmore details
  • 免疫原

    Recombinant polyHistidine tagged fusion protein.

  • 阳性对照
    • Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
  • 常规说明

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

    The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
  • 克隆单克隆
  • 克隆编号HIS-1
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Dot blot 1/2000.
WB 1/2000.

靶标

  • 相关性Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

Anti-polyHistidine antibody [HIS-1] (HRP) (ab49781)参考文献

ab49781 has not yet been referenced specifically in any publications.

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