重组Anti-Polyethylene glycol抗体[RM105] (ab190652)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM105] to Polyethylene glycol
- Suitable for: ICC, WB, ELISA, IHC-P, Flow Cyt, IP
概述
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产品名称
Anti-Polyethylene glycol抗体[RM105]
参阅全部 Polyethylene glycol 一抗 -
描述
兔单克隆抗体[RM105] to Polyethylene glycol -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, ELISA, IHC-P, Flow Cyt, IPmore details -
免疫原
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). (KLH-PEG with terminal methoxy group).
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阳性对照
- PEGylated BSA.
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常规说明
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody (RM105) reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody. This detergent can safely be used in ELISA assays.
Learn more about PEG RabMAb products and kit - PEG RabMAb Product Portal Page
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab190652 was purified from an animal origin–free and protein-free culture supernatant. -
克隆
单克隆 -
克隆编号
RM105 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab190652于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.05 - 1 µg/ml.
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ELISA |
Use a concentration of 0.01 - 0.3 mg/ml.
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IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
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说明 |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 0.05 - 1 µg/ml. |
ELISA
Use a concentration of 0.01 - 0.3 mg/ml. |
IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
靶标
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别名
- PEG antibody
图片
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All lanes : Anti-Polyethylene glycol antibody [RM105] (ab190652) at 0.1 µg/ml
Lane 1 : BSA at 0.2 µg
Lane 2 : PEGylated BSA at 0.2 µg
Lane 3 : BSA at 0.1 µg
Lane 4 : PEGylated BSA at 0.1 µg
Lane 5 : BSA at 0.05 µg
Lane 6 : PEGylated BSA at 0.05 µg
Lane 7 : BSA at 0.02 µg
Lane 8 : PEGylated BSA at 0.02 µg
Lane 9 : BSA at 0.01 µg
Lane 10 : PEGylated BSA at 0.01 µg -
ELISA of three different PEGylated BSA using ab190652 at 0.3 µg/ml, following AP conjugated goat anti-rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab190652 被引用在 1 文献中.
- Geeurickx E et al. The generation and use of recombinant extracellular vesicles as biological reference material. Nat Commun 10:3288 (2019). PubMed: 31337761