重组HRP Anti-Polyethylene glycol抗体[PEG-B-47] (ab202640)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [PEG-B-47] to Polyethylene glycol
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-Polyethylene glycol抗体[PEG-B-47]
参阅全部 Polyethylene glycol 一抗 -
描述
HRP兔单克隆抗体[PEG-B-47] to Polyethylene glycol -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to keyhole limpet haemocyanin. KLH-Polyethylene glycol with a terminal methoxy group.
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阳性对照
- WB: BSA-PEG
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
解离常数(KD)
KD = 2.41 x 10 -9 M Learn more about KD -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
PEG-B-47 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab202640于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200.
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ELISA |
Use at an assay dependent concentration.
Do Not Use TBS As Buffer |
说明 |
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WB
1/200. |
ELISA
Use at an assay dependent concentration. Do Not Use TBS As Buffer |
靶标
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别名
- PEG antibody
图片
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HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640) at 1/200 dilution + BSA-PEG at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutesBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Sandwich ELISA antigen dose-response curve using ab202640.
Primary antibody concentration - ab202640 (500ng/ml and 250ng/ml).
Antigen - MIgG-PEG 5K (0-1000ng/ml).
Coated antibody - Goat anti-Mouse IgG (1000ng/ml). -
Direct ELISA antibody dose-response curve using ab202640. Primary antibody concentration range 0-1000ng/ml, antigen (MIgG-PEG 5K) concentration range 0-500ng/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab202640 被引用在 1 文献中.
- Triplett TA et al. Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nat Biotechnol 36:758-764 (2018). PubMed: 30010674