Anti-Polycystin 1抗体[7e12] (ab74115)

概述

  • 产品名称
    Anti-Polycystin 1抗体[7e12]
    参阅全部 Polycystin 1 一抗
  • 描述
    小鼠单克隆抗体[7e12] to Polycystin 1
  • 经测试应用
    适用于: IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Bacterially expressed fusion protein from the N-terminal leucine rich domain of human Polycystin 1.

  • 表位
    This antibody was produced to the flank-leucine rich repeat-flank region (24-180aa).
  • 阳性对照
    • This antibody gave a positive signal when used to probe a Human Liver, bone marrow, and KidneyTissue section (IHC).
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

应用

Our Abpromise guarantee covers the use of ab74115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      May be an ion-channel regulator. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Involved in adhesive protein-protein and protein-carbohydrate interactions.
    • 疾病相关
      Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1) [MIM:173900]. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000.
    • 序列相似性
      Belongs to the polycystin family.
      Contains 1 C-type lectin domain.
      Contains 1 GPS domain.
      Contains 1 LDL-receptor class A domain.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 17 PKD domains.
      Contains 1 PLAT domain.
      Contains 1 REJ domain.
      Contains 1 WSC domain.
    • 结构域
      The LDL-receptor class A domain is atypical; the potential calcium-binding site is missing.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Autosomal dominant polycystic kidney disease 1 protein antibody
      • Autosomal dominant polycystic kidney disease protein 1 antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • OTTHUMP00000208669 antibody
      • OTTHUMP00000208670 antibody
      • PBP antibody
      • Pc-1 antibody
      • PKD antibody
      • Pkd1 antibody
      • PKD1_HUMAN antibody
      • Polycystic Kidney Disease 1 antibody
      • polycystic kidney disease-associated protein antibody
      • Polycystin 1 Precursor antibody
      • Polycystin-1 antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Serine/threonine-protein kinase D1 antibody
      • transient receptor potential cation channel, subfamily P, member 1 antibody
      • TRPP1 antibody
      see all

    图片

    • IHC image of Polycystin 1 staining in a formalin fixed, paraffin embedded normal human kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • IHC image of Polycystin 1 staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • IHC image of Polycystin 1 staining in Normal Human Bone Marrow formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Overlay histogram showing HEK293 cells stained with ab74115 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74115, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    文献

    This product has been referenced in:
    • Lu H  et al. Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease. Nat Genet 49:1025-1034 (2017). Read more (PubMed: 28530676) »
    • Aguiari G  et al. Novel role for polycystin-1 in modulating cell proliferation through calcium oscillations in kidney cells. Cell Prolif 41:554-73 (2008). Read more (PubMed: 18422703) »

    See all 9 Publications for this product

    客户评价及客户问答

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (kidney nephron)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (6)
    Loading amount
    300 µg
    Specification
    kidney nephron
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Username

    Herr Dr. Andre Kraus

    Verified customer

    提交于 Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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